BIOTECHNOLOGY LABORATORY

Environment

Our biotechnology laboratory supports the consulting department and contributes to maintaining the intense R&D&I activity developed in the company.

ANALYTICAL TECHNIQUES
MICROBIAL ACTIVITY
Environmental laboratory

Technique consisting of measuring the microbiological evolution of a contaminated site in order to assess the effectiveness of a bioremediation treatment and, if necessary, to identify potential improvement actions.

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Plate count

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Enzymatic activity

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ATPmetry

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Respirometry

DNA METABARCODING
DNA metabarcoding

A technique for determining the quantity and diversity of bacteria, archaea, fungi or algae (i.e. a specific taxonomic group) in a sample. This makes it easier to know the microorganisms involved in the decontamination process in soil and water remediation projects.

qPCR
qPCR

Precision technique that quantifies the DNA of a species for:

  • Verify the presence of a key organism in a metabolic pathway.
  • Confirm the absence of a pathogen that could affect a decontamination process.

Some of the species we quantified:

  • Pathogens: Pseudomonas aeruginosa, Escherichia coli, etc.
  • Hydrocarbon degraders
  • Degraders of organochlorine compounds: Dehalococcoides sp.
  • Methane Producers: Acetoclastic Archeae
MICROBIOLOGICAL CULTURES
BIOAUGMENTATION
Laboratory imaging

We isolate and scale microbiological cultures in order to accelerate the bioremediation of a site. For this purpose, KEPLER assesses in each individual case the use of:

  • 1.

    Our collection of microbiological consortia

    Gathered over the years.

  • 2.

    Microorganisms from the site itself

    Where the contamination has occurred. A soil sample is taken from which the autochthonous microbiota is extracted and isolated, which will be concentrated and previously acclimatized to the contaminant.

Process Phases

Identification
Extraction and Isolation
Laboratory Scale Incubation
Scaling in Bioreactors
ESSAYS
FEASIBILITY TESTS
Feasibility

Technique to evaluate the strategy and feasibility of microbiological techniques in a specific project through the identification of possible inhibitors on the microbiology derived from the contaminant product or site.

ECOTOXICITY TESTS
Ecotoxicity

Technique to evaluate the toxicity of a soil or water by observing the growth of test crops or organisms (among others, lactuca sativa).